How to Make a Yeast Starter 101

Category : Technical
Date : May 17, 2023
Author : White Labs & Clawhammer Supply
While White Labs PurePitch Next Generation for Homebrewers comes with the cell count for a foolproof fermentation every batch. By pitching the same cell count every time creates a better likelihood of having the same beer flavor profile and fermentation timeline. Yet there are times when you may want or need to make a yeast starter, such as using an older package or brewing a bigger beer.

RELATED: Get The Yeast Bay Directly From White Labs!

How to Make a Yeast Starter 101-1
A yeast starter allows you to ensure your yeast is in an active state when pitching and will generally lead to a shorter lag time.

Follow along while we break down a step-by-step process of making a starter.

Step 1: Gather Your Supplies

  • 150 grams of DME (Dry Malt Extract)
  • One 2-liter flask
  • Stir Plate
  • Stir Bar
  • Sanitizer 
  • Yeast

Step 2: Sanitize

Sanitization is key when it comes to handling yeast and making starters and beer. A starter is full of nutrients your yeast (and any other microorganisms) need to replicate and begin metabolism which means if there are any other contaminating organisms present, it’s likely to become a problem when fermenting your beer. 

There are many food-grade and no-rinse sanitizers such as StarSan. Check your local homebrew shop to see what’s available. 

How to Make a Yeast Starter 101-2

Step 3: Boil DME

Boil 150 grams of dry malt extract in 1.5 liter of water aiming for about 1.037 specific gravity. Be cautious of scorching the extract as you bring to a boil and allow the solids to dissolve fully. You may choose to add additional yeast nutrients such as White Labs Yeast Nutrient or Servomyces at this stage as well. 

Step 4: Cool and Add Yeast

Ensure the solution is cooled to room temperature. Once cooled, as aseptically as possible, add the yeast. A spray bottle of isopropanol or StarSan is suggested to spray down any surfaces or materials that may come into contact with yeast,  including your hands or gloves. 

How to Make a Yeast Starter 101-3

Step 5: Cover Flask and Turn on Stir Plate

In this step, you want to ensure the yeast continues to get a healthy dose of oxygen and remain aerobic. This will allow the yeast to build their cell walls and prepare for the stressful environment of fermentation. Make sure the flask cover is loose to promote gas exchange. Allow your starter to stir for 24 hours, and then pitch directly to your full volume of wort. You may also refrigerate and then decant but it’s recommended to use your starter as quickly as possible. 
How to Make a Yeast Starter 101-4


  • Use a yeast calculator to understand desired yeast growth and estimated pitch rates
  • A stir plate can increase the growth rate of your yeast, but it's not strictly necessary. You can also shake the flask periodically to keep the yeast in suspension.
  • If you're reusing yeast from a previous batch, sanitize any equipment that will come into contact with the yeast to avoid contamination. 
While a yeast starter may not be necessary with lab-grown yeast, it can be a fun way to manage your yeast culture and ensure an active culture. Happy Brewing!

Check out our friends at Clawhammer Supply for everything you need to start your Yeast Starter